Dojindo,BAPTA-AM/25/B018,BAPTA 是由 Tsien 博士开发的钙选择性螯合剂

2022年4月30日 作者 jinpanbio
Product Description
BAPTA 是由 Tsien 博士开发的钙选择性螯合剂。 它的 logKCa=6.97 和 logKMg=1.77。 基本螯合单元类似于EGTA,但两个脂肪族氮原子被芳香族氮原子取代。 因此,BAPTA 在生理 pH 下不被质子化。 BAPTA 具有 pKa3=5.47 和 pKa4=6.36。 该性质表明去质子化步骤不包括在其钙络合步骤中,并且它具有比EGTA更高的络合率,因为它不受质子干扰的影响。 BAPTA-AM 是 BAPTA 的乙酰氧基甲酯衍生物,可以使用 AM 方法轻松加载到细胞中。 BAPTA-AM 可用于控制细胞内钙浓度。

Hydrolysis of AM ester

1. R. Y. Tsien, New Calcium Indicators and Buffers with High Selectivity against Magnesium and Protons: Design, Synthesis, and Properties of Prototype Structures. Biochemistry. 1980;19:2396-2404.2. R. Y. Tsien, A Non-disruptive Technique for Loading Calcium Buffers and Indicators into Cells. Nature. 1981;290:527-528.3. J. I. Korenbrot, et al., The Use of Tetracarboxylate Fluorescent Indicators in the Measurement and Control of Intracellular Free Calcium Ions. Soc Gen Physiol Ser. 1986;40:347-363.4. S. M. Harrison, et al., The Effect of Temperature and Ionic Strength on the Apparent Ca-affinity of EGTA and the Analogous Ca-chelators BAPTA and Dibromo-BAPTA. Biochim Biophys Acta. 1987;925:133-143.5. E. W. Gelfand, et al., Dissociation of Unidirectional Influx of External Ca2+ and Release from Internal Stores in Activated Human T Lymphocytes. Eur J Immunol. 1990;20:1237-1241.6. J. P. Kao, et al., Active Involvement of Ca2+ in Mitotic Progression of Swiss 3T3 Fibroblasts. J Cell Biol. 1990;111:183-196.7. M. L. Schubert, et al., Functionally Distinct Muscarinic Receptors on Gastric Somatostatin Cells. Am J Physiol. 1990;258:G982-G987.8. Y. Tojyo, et al., Inhibitory Effects of Loading with the Calcium-chelator 1, 2-Bis(o-aminophenoxy)ethane-N, N, N E N Etetraacetic acid (BAPTA) on Amylase Release and Cellular ATP Level in Rat Parotid Cells. Biochem Pharmacol. 1990;39:1775-1779.