Dojindo,ARP醛反应探针/25/A305,醛反应探针 (ARP) 因其醛特异性反应性

2022年4月29日 作者 jinpanbio
醛反应探针 (ARP) 因其醛特异性反应性而被用于检测 DNA 中的脱碱基位点(AP 位点、脱嘌呤/脱嘧啶位点)。 ARP 与醛基和酮基反应,并将生物素添加到 DNA 中的 AP 位点。 然后使用过氧化物酶标记的抗生物素蛋白和氧化显色染料检测生物素标记的 DNA。 ARP 方法已被用于检测 1×104 个 DNA 核苷酸中少于 1 个 AP 位点。 Dojindo 提供 DNA 损伤定量试剂盒(产品代码:DK02),其中包含确定每 1×105 个碱基对中 1 到 40 个 ARP 位点所需的所有试剂和组分。 ARP 极易溶于水,储备液可在 4ºC 下储存一年而不会显着降低反应性。

Labeling Procedure for DNA Abasic Site1. Prepare 10 mM ARP with water.2. Adjust the DNA concentration to 100 μg per ml with TE buffer (pH 7.4).3. Mix equal volumes of the DNA and ARP solutions and incubate at 37ºC for 1 hour.4. Isolate the ARP-labeled DNA using either ethanol precipitation or membrane filtration tube.5. Dissolve the DNA pellet in TE to prepare 10-100 μg per ml solution.

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