Dojindo,用于线粒体单线态氧成像的 Si-DMA/2/MT05
2022年4月27日马岛等人。合成了一种由含硅罗丹明和蒽部分组成的新型远红荧光探针，即 Si-DMA，分别作为生色团和 1O2 反应位点。在 1O2 存在下，由于蒽部分 (1),(2) 形成内过氧化物，Si-DMA 的荧光增加了 17 倍。在七种不同的 ROS 中，Si-DMA 能够选择性地检测 1O2（图 3）。此外，Si-DMA 能够用 5-氨基乙酰丙酸 (5-ALA)（血红素的前体）实时显示线粒体中原卟啉 IX 生成 1O2。
Usage ExampleFluorescence microscopic detection of 1O2 in HeLa cells after added 5-aminolevulinic acid (5-ALA)1. HeLa cells (2.4×105 cells/ml, Hanks’ HEPES buffer 200 μl) were seeded on a μ-slide 8 well (Ibidi) and cultured at 37oC in a 5%CO2 incubator overnight.2. Cells were washed with 200 μl Hanks’ HEPES buffer twice.3. 150 μg/ml of 5-ALA in Hanks’ HEPES buffer was added to the μ-slide and cultured at 37oC in a 5%CO2 incubator for 4 hours.4. Cells were washed with 200 μl Hanks’ HEPES buffer twice.5. 200 μl of Si-DMA working solution (40 nmol/L) was added and cultured at 37oC in a 5%CO2 incubator for 45 minutes.6. Cells were washed with 200 μl Hanks’ HEPES buffer twice.7. 200 μl Hanks’ HEPES buffer was added and observe the cells under a fluorescence microscope.