Dojindo,2,3-二氨基萘（不检测）/10/D418,Griess 法是检测 NO 浓度的方法

Reaction of 2,3-Diaminonaphthalene with NO₂

NO₂ Assay Using 2,3-Diaminonaphthalene (DAN)1. Dissolve 50 μg DAN in 1 ml 0.62 M HCl to prepare 0.31 mM DAN solution.^a)2. Mix 10 μl DAN solution with 100 μl NaNO₂ solution (0-10 mM) or sample solution. Incubate the mixture at room temperature for 10-15 minutes.3. Add 5 μl 2.8 M NaOH solution to the reaction solution.^b)4. Dilute 100 μl of this solution with 4 ml water, followed by fluorescent measurement with excitation wavelength at 365 nm and emission wavelength at 450 nm.5. Prepare a calibration curve using this data where the X-axis is NaNO₂ concentration and the Y-axis is fluorescence intensity. Then, use this calibration curve to determine the NO₂ concentration of the sample solution.

a) Acidic conditions are required for a rapid reaction.b) Basic conditions (pH 10 or higher) are required for a high fluorescence signal.

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