lonza,PT-3001,PT-2501,CC-3232,MSCGM™ 间充质干细胞生长培养基 BulletKit™

2022年4月13日 作者 jinpanbio
货号: PT-3001

Culture system containing MSCBMTM Basal Media (PT-3238) and MSCGMTM SingleQuots Supplement Kit (PT-4105) required for proliferation of human bone marrow derived mesenchymal stem cells.

包含人骨髓来源的间充质干细胞增殖所需的 MSCBMTM 基础培养基 (PT-3238) 和 MSCGMTM SingleQuots Supplement Kit (PT-4105) 的培养系统。

Product Overview

Stem cells possess the unique ability to proliferate as an undifferentiated cell type, then, when properly stimulated, differentiate into multiple lineages of cells. Lonza’s MSCGMTMhuman Mesenchymal Stem Cell Growth BulletKitTM Medium is a serum containing medium designed to proliferate human bone marrow derived mesenchymal cells in an undifferentiated state.

The serum used in Lonza’s MSCGMTM Human Mesenchymal Stem Cell Growth Medium BulletKitTM Medium is specifically screened to ensure that the serum neither promotes spontaneous differentiation of cells nor inhibits cell differentiation to osteogenic, chondrogenic, or adipogenic lineages (when properly stimulated with an appropriate differentiation media).

The MSCGMTM Human Mesenchymal Stem Cell Growth BulletKitTM Medium is offered as a BulletKitTM Medium (catalog no. PT-3001) which includes both the basal media and the necessary supplements for proliferation of human bone marrow derived mesenchymal stem cells. The basal medium (MSCBMTMhMSC Basal Medium; catalog no. PT-3238) and the necessary supplements (MSCGMTMhMSC SingleQuot Kit; catalog no. PT-4105) can also be purchased individually. Trypsin/EDTA (catalog no. CC-3232): Lonza’s recommended Trypsin/EDTA for all strongly adherent primary cells including normal human articular chondrocytes (NHAC-Kn), human bone marrow derived mesenchymal stem cells (hMSC), and dental pulp stem cells (DPSC).

Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.

干细胞具有作为未分化细胞类型增殖的独特能力,然后在适当刺激时分化成多个细胞谱系。 Lonza 的 MSCGMTMhuman 间充质干细胞生长 BulletKitTM 培养基是一种含有血清的培养基,旨在增殖处于未分化状态的人骨髓来源的间充质细胞。

Lonza 的 MSCGMTM 人间充质干细胞生长培养基 BulletKitTM 培养基中使用的血清经过专门筛选,以确保血清既不促进细胞的自发分化,也不抑制细胞分化为成骨、软骨或脂肪谱系(当用适当的分化培养基适当刺激时) .

MSCGMTM 人间充质干细胞生长 BulletKitTM 培养基以 BulletKitTM 培养基(目录号 PT-3001)的形式提供,其中包括基础培养基和人骨髓衍生间充质干细胞增殖所需的补充剂。基础培养基(MSCBMTMhMSC 基础培养基;目录号 PT-3238)和必要的补充剂(MSCGMTMhMSC SingleQuot 试剂盒;目录号 PT-4105)也可以单独购买。胰蛋白酶/EDTA(目录号 CC-3232):Lonza 推荐用于所有强粘附原代细胞的胰蛋白酶/EDTA,包括正常人关节软骨细胞 (NHAC-Kn)、人骨髓间充质干细胞 (hMSC) 和牙髓干细胞(DPSC)。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时,Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改,包括使用替代培养基、试剂或方案,都将使细胞和培养基性能保证失效。

Content & Storage

Content

1 x MSCBMTM Basal Media (PT-3238), 500 mL 

1 x MSCGMTM SingleQuotsTM Growth Supplement Kit (PT-4105)

 

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