lonza,CC-2547,V5SP-2010,SAEC – 人类小气道上皮细胞
2022年4月12日Cryopreserved ampule of Small Airway Epithelial Cells (SAEC) containing ≥500,000 cells.
Product Overview
Lonza’s Human Small Airway Epithelial Cells (SAEC) are isolated from normal human lung tissue the distal portion of the lung in the 1mm bronchiole area.
Cryopreserved SAECs or Alveolar Cells are guaranteed through 15 population doublings and stain positive for cytokeratin 19. SAECs or Alveolar Cells labeled CC-2547S are suitable for air-liquid interface studies and are guaranteed for differentiation marked by cilia formation, mucin production, and TEER levels. Cells from various diseased donors are also available.
All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Lonza 的人小气道上皮细胞 (SAEC) 是从正常人肺组织中分离出来的，位于 1mm 细支气管区域的肺远端部分。
冷冻保存的 SAEC 或肺泡细胞通过 15 次群体倍增和染色呈细胞角蛋白阳性 19 得到保证。标记为 CC-2547S 的 SAEC 或肺泡细胞适用于气液界面研究，并保证以纤毛形成、粘蛋白产生和 TEER 水平为标志的分化 . 还可以获得来自各种患病供体的细胞。
所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。
Benefits
- All cells test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- Certificate of Analysis provided for every cell lot
Content & Storage
Content
1 x Cryopreserved ampule SAEC containing ≥500,000 cells
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Co-culture Experiments with Lonza’s Small Airway Epithelial Cells and SAGM™ BulletKit™ Media
Data Courtesy of Furukawa, et al. Lonza’s SAEC cells were co-cultured with primary normal mammary epithelial cells, breast cancer cell lines MCF-7 and MDA-MB-231 in Lonza’s SAGM™ BulletKit™ Growth Media. Breast cancer cell lines showed differences in morphology and proliferation rates in the presence of small airway epithelial cells. The image above shows phase contrast imaging with a fluorescent (red) overlay of MCF-7 cell line. MCF-7 cell lines survived as tight clusters in SAGM™ Media. When co-cultured with Lonza’s primary SAECs, MCF-7 cells demonstrated enhanced growth all the while staying clustered.