LI-COR 标准化的 β-肌动蛋白小鼠单克隆抗体,LI-COR P/N 926-42212

2022年3月15日 作者 jinpanbio

Reagents > β-Actin Mouse Monoclonal Antibody for Normalization,用于标准化的 β-肌动蛋白小鼠单克隆抗体

β-Actin Mouse Monoclonal Antibody for Normalization

The β-actin primary antibody can be used as an internal loading control (ILC) for normalization.

The expression of β-actin, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.

Once validated, β-actin primary antibodies can be used for the detection of β-actin when performing two-color detection.

Detect β-Actin Mouse Primary Antibody with IRDye® Goat anti-Mouse or IRDye Donkey anti-Mouse secondary antibodies.

Other options for housekeeping protein normalization

β-肌动蛋白一抗可用作内部上样对照 (ILC) 以进行标准化。

应验证 β-肌动蛋白或任何管家蛋白 (HKP) 的表达,以确保其表达在实验条件下不会改变。

经验证后,β-actin 一抗可用于进行双色检测时对 β-actin 的检测。

使用 IRDye® Goat anti-Mouse 或 IRDye Donkey anti-Mouse 二抗检测 β-Actin Mouse Primary Antibody。

管家蛋白质标准化的其他选择

Reactivity and Specificity

β-Actin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.

Do not aliquot the antibody.

β-肌动蛋白抗体以 10 mM HEPES (pH 7.5)、150 mM NaCl、100 µg/mL BSA、50% 甘油和 <0.02% 叠氮化钠的形式提供。

不要等分抗体。

Properties β-Actin Mouse Monoclonal Antibody (P/N 926-42212)
Species Cross-Reactivity Human, mouse, rat, monkey, hamster
Target Molecular Weight 45 kDa
Isotype Mouse IgG2b
Specificity/Sensitivity Detects endogenous levels of β-actin protein
Immunogen A synthetic peptide (KLH-coupled) that corresponds to the residues near the amino-terminus of human β-actin
Tested Applications Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F)

RRID

  • P/N 926-42212: RRID AB_2756372

Actin Used for Normalization

β-Actin Mouse Monoclonal Antibody for Normalization
Presence of Smad2/3 in Xenopus embryo lysates. Xenopus embryos were injected with Smad2 RNA and treated with noggin, truncated BMP receptor (tBR), truncated activin receptor (XAR) or BMP4. Lysates were electrophoresed on 12.5% SDS-PAGE gel and proteins were transferred onto PVDF membrane. Membrane was probed for Smad2/3 and β-actin expression using rabbit anti-smad2/3 and mouse anti-actin monoclonal antibody (P/N 926-42212) followed by detection with IRDye 800CW Goat anti-Rabbit IgG (P/N 926-32211) and IRDye 680RD Goat anti-Mouse IgG (P/N 926-68070). Contributed by Kimberly Wong, SUNY Upstate Medical Center, NY, United States.
非洲爪蟾胚胎裂解物中存在 Smad2/3。 非洲爪蟾胚胎被注射 Smad2 RNA 并用 noggin、截短的 BMP 受体 (tBR)、截短的激活素受体 (XAR) 或 BMP4 处理。 裂解物在 12.5% SDS-PAGE 凝胶上进行电泳,并将蛋白质转移到 PVDF 膜上。 使用兔抗 smad2/3 和小鼠抗肌动蛋白单克隆抗体 (P/N 926-42212) 检测膜的 Smad2/3 和 β-肌动蛋白表达,然后用 IRDye 800CW 山羊抗兔 IgG (P/N 926) 检测 -32211) 和 IRDye 680RD 山羊抗小鼠 IgG (P/N 926-68070)。 由美国纽约州纽约州立大学北部医学中心的 Kimberly Wong 提供。