LI-COR 标准化的 β-肌动蛋白兔单克隆抗体,LI-COR P/N 926-42210

2022年3月15日 作者 jinpanbio

Reagents > β-Actin Rabbit Monoclonal Antibody for Normalization,用于标准化的 β-肌动蛋白兔单克隆抗体,P/N 926-42210

β-Actin Rabbit Monoclonal Antibody for Normalization

The β-actin primary antibody can be used as an internal loading control (ILC) for normalization.

The expression of β-actin, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.

Once validated, β-actin primary antibodies can be used for the detection of β-actin when performing two-color detection.

Detect β-Actin Rabbit Primary Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.

Other options for housekeeping protein normalization

β-肌动蛋白一抗可用作内部上样对照 (ILC) 以进行标准化。

应验证 β-肌动蛋白或任何管家蛋白 (HKP) 的表达,以确保其表达在实验条件下不会改变。

经验证后,β-actin 一抗可用于进行双色检测时对 β-actin 的检测。

使用 IRDye® Goat anti-Rabbit 或 IRDye Donkey anti-Rabbit 二抗检测 β-Actin Rabbit Primary Antibody。

管家蛋白质标准化的其他选择

Reactivity and Specificity

β-Actin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.

Do not aliquot the antibody.

Properties β-Actin Rabbit Monoclonal Antibody (P/N 926-42210)
Species Cross-Reactivity Human, mouse, rat, monkey
Target Molecular Weight 45 kDa
Isotype Rabbit IgG
Specificity/Sensitivity May cross-react with γ-actin (cytoplasmic isoform)
Immunogen A synthetic peptide (KLH-coupled) that corresponds to the residues near the amino-terminus of human β-actin
Tested Applications Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F)

RRID

  • P/N 926-42210: RRID AB_1850027