LI-COR IRDye® 山羊抗兔,IRDye 驴抗兔二抗,检测 β-微管蛋白兔多克隆抗体

2022年3月14日 作者 jinpanbio

Reagents > β-Tubulin Rabbit Polyclonal Antibody for Normalization,用于标准化的 β-微管蛋白兔多克隆抗体

β-Tubulin Rabbit Polyclonal Antibody for Normalization

The β-Tubulin primary antibody can be used as an internal loading control (ILC) for normalization.

The expression of β-tubulin, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.

Once validated, β-tubulin primary antibodies can be used for the detection of β-tubulin when performing two-color detection.

Detect β-Tubulin Rabbit Polyclonal Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.

Other options for housekeeping protein normalization

β-微管蛋白一抗可用作内部上样对照 (ILC) 以进行标准化。

应验证 β-微管蛋白或任何管家蛋白 (HKP) 的表达,以确保其表达在实验条件下不会改变。

经验证后,β-微管蛋白一抗可在进行双色检测时用于检测 β-微管蛋白。

使用 IRDye® 山羊抗兔或 IRDye 驴抗兔二抗检测 β-微管蛋白兔多克隆抗体。

管家蛋白质标准化的其他选择

Reactivity and Specificity

β-Tubulin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.

Do not aliquot the antibody.

Properties β-Tubulin Rabbit Polyclonal Antibody (P/N 926-42211)
Species Cross-Reactivity Human, mouse, rat, monkey, bovine
Target Molecular Weight 55 kDa
Isotype Rabbit IgG
Specificity/Sensitivity Detects endogenous levels of total β-tubulin protein. Does not cross-react with recombinant α-tubulin.
Immunogen A synthetic peptide (KLH-coupled) that corresponds to the sequence of total β-tubulin, and does not cross-react with recombinant α-tubulin
Tested Applications Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F)

蛋白质印迹 (WB)、免疫组织化学 (IHC)、免疫荧光 (IF)、流式细胞术 (F)

RRID

  • P/N 926-42211: RRID AB_1850029

β-Tubulin Detected in Various Cell Lines

β-Tubulin Rabbit Polyclonal Antibody for Normalization
β-Tubulin detected with β-Tubulin primary antibody (P/N 926-42211) in HeLa, NIH/3T3, and COS7 cell lysates.